nm0187: so on the handout you've got this and i don't i'm not going to go through every lecture and and start talking about it but [0.2] you have got this and hopefully you'll see this develops come along so we'll cover the [0.2] the general introduction [0.2] this week [0.2] and then there's the main [0.2] types of breeding schemes [0.2] er and then [0.2] some more of the of the techniques and backgrounds [0.3] er and ending up [0.3] with [1.1] the releasing of varieties and all the things that go with that about patenting plant breeders' rights and [0.5] a little bit about the ethics er [1.0] particularly of course the [0.2] G-M-Os and this sort of thing [0.9] okay [0.6] so hopefully that sort of structure will make sense as we [0.2] as we go along [1.8] the [1.3] the other side of your sheet is trash you can [0.2] pretty well ignore [0.2] it's about the er [0.7] the posters and i've changed it slightly and i'll give you another sheet [0.4] er [0.3] just now [0.2] but but basically [0.2] you've got [1.0] from this week after the lecture i'll [0.2] give you a a a list and some information to pick a topic [0.7] and you each pick one topic [0.2] you'll do your poster and your essay on that one topic [0.4] you'll then [0.2] prepare it during the time including using er [0.2] the computers over in P-S-L to to produce the first draft [0.3] and then in weeks eight and nine [0.7] you'll present the posters [0.5] before we'll ask you questions about and et cetera and hopefully learn from them [0.4] er [0.4] and [0.4] then [0.3] week ten [0. 6] if there's anything to pick up we can do [0.6] okay [1.0] yeah [0.3] sm0188: er so we [0.3] give in the posters in week seven [0.2] er [0.6] nm0187: er yeah you'll need to give us the er the posters i'll explain on a disk [0.2] in week seven so that we can [0.7] get it er in a state that you can present it on the on the next week [0.6] er [0.2] and then the er [0.3] the essays need to come in [0.4] in week eight on the same subject area [0.2] i'll go through it a bit more [0.2] er [0.3] just now okay [0.3] we'll come back to that [1.3] okay so that's the main [0.9] outline of things [0.7] what i want to do is to start off by giving you some [0.3] of the [0.6] general introduction to plant breeding itself [0.7] okay [0.4] now if you do have questions then please do feel free to ask them as we [0.2] go along and i will try and remember at the end of each lecture if i don't shout at me [0.3] to please ask whatever questions you want to okay [0.5] but [0.4] otherwise just ask as we go along [8. 9] okay [3.5] as i said i'm not sure if you were all here when i said [0.2] what i'll try and do is to try and give you handouts of all the overheads so er so you shouldn't have to scribble them down too much but [0.4] er [0.4] you may want to add [0.8] more information in various ways [11.8] oh the other important thing to tell you despite what it says on your timetable [0.7] next week the lecture is also here [1.3] because they haven't finished the building [laugh] [0.5] okay [0.2] so [0.3] so next week turn up here again [6.4] [hum] [1.0] [hum] [7.6] okay [1.9] what i'd like to s-, [0.5] start by doing and i hope you don't [0.3] regard this as too trivial [0.3] is [0.2] just to put plants in context a little bit [0.4] as i s-, [0.2] as i see them [1.0] certainly as far as [0.3] humans are concerned [1.5] what you have to remember is that we are completely dependent on plants [1.2] okay [0.4] we're not completely dependent on animals we are completely dependent on plants [0.6] we either eat [0.3] them [0.3] or eat [0.5] the thing that ate them [0.9] okay [1.3] so whatever happens [0.3] and whenever we're talking about [0.7] even growing crops or whatever [0.3] er or whether we're growing ornamental plants we are completely dependent on them in one one [0.5] sense or another [1. 3] they are the only route [0.4] or basically the only route that we can go [0. 2] from the inorganics [0.4] C-O-two et cetera [0.4] to [0.3] the organic [0.2] and use [0.4] the products [0.3] okay [0.4] so they're extremely important in their own right never mind what we're going to do or how we're going to exploit them [1.0] and food is not the only product we get from [0.4] from plants [0.3] i tend to to talk quite a bit [0.2] about [0.4] food crops [0.4] but that's not because they're the only ones it's just it's it's slightly easier to talk about and they're ones i'm more familiar with [0.5] but of course there are all the ornamentals for a start which are [0.3] important certainly commercially important [0.4] but there are also a whole range of other things that we get from plants [0.6] drugs for instance clothing [0.7] paper rubber dyes [0.2] all these sort of products [0.4] are come from plants and are ones which can be subject of course to plant breeding [0.4] to crop improvement et cetera [2.6] so [0.2] they're essential element to sustaining [0.5] human life [0.9] and although other organisms has various roles they really are s-, central [0.3] er [0.2] to what we're doing and for our survival [2.4] so it's important when we go on to talk about cropping you do [0.3] realize that central [0.2] role and [0.3] as i say a lot of the time we may be talking about quite narrow things [0.3] but [0.2] you need to bear in mind the broader context in which plants are [0.3] are there [0.7] and the ways that we're going to be exploiting them or could exploit them [1.8] okay so having [0.4] said that you'll not be [0.5] surprised [0.4] if [0.3] when we ask the question how long has plant breeding been going on [0.2] the answer is as long as we've been [0.4] practising settled [0.2] agriculture of any [0.3] shape or form [0.6] okay [1.0] [cough] [0.5] so for instance if a farmer went out and collected seed [1.1] grew them and they did relatively well [0.3] the chances are he'd go back to the same place to collect seed or use the seed he'd grown [0.8] okay [0.7] if a neighbour [0.4] had [0.9] a crop that was growing particularly well you'd not be surprised if his neighbours [0.2] had a-, [0.4] acquired seed [0.4] or were given it [0.3] by him in one way or another [0.2] they would get it and particular types [0.3] would therefore tend to be [0.2] er [0.3] multiplied [0. 7] and so by this picking of the best [0.9] then [0.6] you'd start to get qu-, [0.3] fairly quickly at least over a reasonable [0.2] times [0.3] types that were different from the wild [0.5] er ones growing around them [0.2] in other words you'd start to get domesticated forms [0.8] which were [0.2] different from [0.4] the general population of of the wild plants around [1.6] and these would then start to form almost exclusively the basis for [0.6] the p-, [0.3] plants the crops that were cultivated [0.2] they would only use those because they wouldn't get such a good yield or they wouldn't get the right sort of [0. 4] er [0.4] product from them [0.7] from the the wild ones so they'd grow these [1.2] so certainly before Mendel's experiments were reported most of the crop plants had been domesticated [1.3] so most of the crops as we recognize them today okay they may look s-, [0.6] different to some extent but you'd recognize them as crop [0.4] were all carried out before we had any knowledge whatsoever of genetics [1.2] so this was basically a lot of it carried out [0.2] by people who were simply growing producing things and knowing what they wanted to to grow and produce [1.4] so i talk about plant breeding as a science and we do put science into it [0.2] but you do have to remember that a lot of what was achieved [0.2] was before we even recognized this so [0.4] i mean that is is the case and i'm not [0.3] er [0.3] in any way belittling their contribution it's very very big [0.7] and they did it without [0.2] the sort of knowledge that mostly we're going to talk about [1.8] so it kind of puts us in context a bit [1.4] [cough] [1.1] okay [0.3] so moving on from there [0.5] to ask really the next [0.2] general question you might ask what would be the objectives [0.2] of [0.2] the crop breeder [2.0] [cough] [1. 5] well [0.2] in many ways they're still the same objectives in broad terms that were originally the driving force [0.6] and a major one is increasing productivity [1.0] certainly in most of the crop plants that is still a major consideration [1.5] the desire and need to produce more [0.4] per unit area [0. 8] is really just as it's always been [0.7] in Europe [0.4] we do get and in a number of the other developed countries [0.4] we do get surpluses of some crops [0.6] and we do get [0.3] quite a lot of discussion about [0.4] whether this means we should not [0.3] produce [0.8] cultivars or types that are more highly productive et cetera [0.8] but if you think about it there's really no industry no [0.2] i-, system [0.3] by which [0.4] increased [0.2] efficiency [0.3] and productivity is not sensible [0.8] you'd be better to go for increased productivity [0.3] but [0.6] growing a smaller area than you would the other way around [0.2] profitability et cetera is still an important consideration [1. 5] but i'm not suggesting that should be the only one i'm not suggesting that we should ignore things like the environmental impact that we're having [0.5] this is something else that we have to consider [0.5] but if we're talking for instance about low input [0.2] production [0.3] then we do need to have cultivars which are suited [0.3] to that [0.5] there's no point thinking you can just take any cultivar and put it in a low input system [0.6] you got to look for ones [1.1] that grow well for instance [0.5] without high nitrogen inputs [0.6] that possibly do grow quite [0.3] well competitively with weeds [0.4] that are [0.2] resistant to many of the pests and diseases if you're not going to control these [0.2] by chemical means [1.3] okay [0.3] so the need [0.9] for [0.4] the increase in production is still there and is still a major driving force [0.3] the other thing which you need to bear in mind this is that much of the world this is not the case [0. 4] they do not have an over-production [0.4] that's absolutely sure [0.8] and you can talk about the total world production and if you spread it out what would happen but that's not reality [0.9] most of the food crops have to be grown [0.3] locally [0.4] to be [0.2] used effectively [0.4] so many countries there's still a very real need [0.2] to feed [0.2] the people that are there [0. 9] and if you look at the projections for the world population you can see this is not something that's going to go away very quickly or very easily [4.9] so one of the objectives [0.6] of plant breeding [0.4] is really to produce lines cultivars or varieties and i use those [0.4] terms interchangeably [0.8] that are er better adapted [0.2] to the particular [0.3] environmental [0.7] conditions under which they're expected to grow [1.7] okay so there's not a universal [0.3] type of cultivar it's got to be [0.4] cultivars that are [0.3] suited to the particular conditions under which they're going to grow [0.9] now these might be climatic conditions which clearly [0.3] vary quite dramatically [0.5] around the world they might be [0.3] er different day lengths [0.3] they might be different temperatures [0.3] and just as easily they might be different farming practices different husbandry techniques [1.0] these are the sort of considerations [0.9] that you need when you're thinking about [0.3] what they're going to be adapted to [2.4] so one of the questions that [0.3] arises what do i really mean by better adapted [0.5] er [0.9] and mostly in plant breeding terms this has tended [0.5] previously [0.3] to simply mean whether they produce more [0.2] crude yield [0.7] whether you get more [0.3] tatties on the bottom of your plant whether you get more [0.2] grains of wheat [2.3] whether you get more [0.4] tea leaves [0.2] off the bushes et cetera [0.3] but this has been modified [0.5] recently to be [0.3] much more sensible [0.2] or at least i think [1.0] it's more sensible [2.8] first of all the main [0.4] change has been much more [0.3] towards usable yield rather than total yield [1.5] so this is the amount [0.5] that you can actually [0.3] you get [0.5] for the purpose that you're considering so [0.2] the amount that you actually get to eat off it the amount you get [0.3] processed from it [0.8] the amount that you [0.3] can actually use after storage [1.3] these sorts of considerations [1.2] so it starts bringing in [0.3] important other factors like the nutritional [0.4] er value [0.5] of the product [0.5] like [0.5] the er ability to withstand storage diseases [1.7] like the reduction in [0.2] waste [0.6] for example if you take potatoes if you peel them [0.3] how much do you lose [0.5] all these sorts of considerations [0.3] now impose [0.4] themselves [0.3] on usable yield [0.4] rather than simply [0.6] crude yield [1. 2] okay [1.8] the next consideration that has become [0.4] much more recognized [0.3] is the need for stability of yield [1.7] now in countries like this mostly or quite a lot of this has been achieved [0.3] by [0.7] having inputs [0. 8] chemical inputs mostly by using fertilizers [0.3] by using sprays [1.2] so that is one way of stabilizing the the at least the gross [0.2] yield [0.5] on a year by year basis [1.5] but the other [0.4] way that's also been done is to look at the [0.3] er [1.2] plant breeding and the resistance to [0.4] things like biotic and abiotic stresses [0.5] to cut down the year to year [0.3] variation [0.6] i think it's fairly obvious particularly if you're depending on a crop if one year [0.2] it yields a lot [0.5] the next year it yields a lot and the one after [0.8] not very much at all [0.4] the average of those three years might look all right and you might say that's quite a good variety [0.3] but in realistic terms if you haven't got anything to eat in the third year is is kind of a bit tricky [0.8] okay [0.6] so although it seems obvious it's not something that's been too highly [0.3] er [0.7] stressed before [0.8] so as i say one of the ways round this is to look very much at things like disease [0.4] resistance and [0.2] tolerance to [0.2] abiotic stresses temperature [0.5] er drought [0.4] water-logging [0.2] any of these types of things [3.2] the next thing that [0.4] as i say has been having increasing emphasis is the quality of the product [2.3] clearly one of the important parts in a food crop is the nutritional [0.3] quality itself [0.2] but also aspects like taste [0.7] i'm sure you've all heard the stories of how the new [0.5] varieties do not taste anything like the old ones and we've got rid of all the [0.4] good qualities of these and to some extent it's it's true [0.4] er [0.5] but it's certainly something that is much more er [0.3] focused on [0.2] now [0.4] there's also things like [0.2] the calorific value [0.3] er [0.4] co-, protein content [0.3] fat level vitamin concentration [0.4] all these things are aspects [0.4] that do vary [0.4] between different genotypes and you can select for [0.8] within the [0.4] within the crop [2.1] [cough] [0. 7] then there's the environmental impact which we've [0.3] touched on before [2.9] agriculture [0. 3] when it's practised [0.2] has raised [0.5] quite a considerable debate in in quite a number of ways [0.4] and [0.2] in most developed countries we do the work for intensive [0.5] agricultural production [1.2] there is very little [0. 5] in this country which you could call a natural environment [0.5] it depends er [0.3] to a despite [0.6] people protesting [0.4] about [0.3] er [0.5] the effects on the countryside the countryside is almost completely agricultural [0. 2] production it's certainly [0.3] framed and [0.3] a product of agricultural production [0.7] so we do have to [0.2] to bear this in mind [0.2] when you look at other countries and you talk about them [0.3] not destroying their rainforests and all the rest of it which i [0.6] fully sympathize with [0.2] you have to remember that's exactly what we have done [0.7] we have taken out all our forests [0.4] and put in agriculture [1.5] but it's something that er er say we need to to think [0.4] carefully about we need to think about inputs [0.4] such as do we need high fertilizer levels do we need chemicals et cetera one of the answers [0.3] is undoubtedly from plant breeding [1.4] if we can produce yield without such a high level of [0.2] fertilizer [0. 8] if we can stabilize yield without having to spray [0.2] pesticides then clearly [0.3] that is something we should [1.4] or we could go for and to a certain extent it is being done [2.0] again you have to [0.3] look [0.5] at it in the context that you expect the thing to be grown [1.0] if it's going to be grown in a high input system then you will have [0.8] cultivars that will respond and grow in that situation [0.3] if you're looking at low input then it must be in that if you're looking in a developing country [0.5] you have to look [0.4] at what condition and husbandry practices they're going to do and what effect that's going to have [7.3] as i said one of the primary aims [0.4] is to produce varieties which are better adapted [0.8] but the second part of that [0.4] is to the particular environmental conditions under which they're expected to grow [3.5] to produce varieties that [0.6] are growing under [0.5] these conditions it's quite an easy thing to overlook [0.7] you do have to think of a whole series of th-, of [0.5] things depending on how broadly you expect the [0.8] the two that you're producing to grow [1.4] what are the soil conditions [0.8] what sort of rainfalls [1.5] what sort of [0.4] basic environmental conditions such as er [0.2] hours of sunlight [0.5] are you going to [0.3] be exposed to [1.1] but very importantly is what type of [0.4] husbandry practice what type of farming system [0.5] basically is it going to be [0.4] to be grown under [0. 7] mostly of course [0.3] breeders are producing for [0.2] large-scale [0.2] production they are not usually [0.3] selecting for smallholder production [1. 7] but nevertheless there are quite a variety [0.3] of different ways [0.2] in which [0.5] you can grow crops [1.5] okay so this [0.5] is something which is important to bear in mind [3.5] so the [0.6] objectives of the plant breeder are to increase usable yield [0.3] increase stability [0.4] make the crop more nutritive reduce ecological disadvantages [0.5] and produce types [0.3] that basically suit [0.2] the growing conditions and the needs of the people who are growing them [2.3] there are however as i [0.4] pointed out at the start were the number of other factors that you need to worry about and the most difficult one [0.5] as i said from the start is that you're going to have to predict [0.6] what [0.3] this is going to be like in the future not what it is today [1.0] probably as i said to you the minimum is probably about seven years [0.3] in the future [2.3] now [0.2] in some ways environmentally [0.4] that might not be too bad even with global warming [0.2] seven years is a moderately short [0.6] time scale so you could maybe [0.3] er [0.4] do a reasonable job on that [1.6] but how are you going to predict what for instance the political situation is going to be [1.0] what is going to be [0.4] the position with regard for instance the subsidies or [0.5] what have you [0.2] what crops will [0.3] farmers want to grow [3.8] what will the end-user want [2. 0] you only have to think of the changes that have occurred in [0.5] food consumption [0.3] in this country or [0.3] any of the developing ones to see what happens [0.7] in the in the developed ones i mean in the developing ones the same thing is true [0.5] what [0.2] is going to be the requirements when i started [0.4] as a plant breeder [1.0] as a potato breeder at least [0.4] then [0.4] the selection was all for a medium-size potato tuber [0.2] that was what were required by the housewife and all the rest of it [0.4] all the small ones used to throw away [0.5] and the great big ones were useless and they were also [0.3] given for [0.2] animal feed [0.8] because there was no [0.2] real use for them so all the breeding was for [0.2] this size tuber [0.7] within a few years [0.2] of course the big tubers [0.3] started getting a premium because they're used for bakers [0.6] and you can sell those and throw the rest away if you want to although you don't you use them so there's a premium on those [0.3] and they even sell the small ones then they pretend they're Earlies [0.3] and sell those at a premium [0.6] so the whole thing reversed within [0.2] really a few years [0.2] now as a plant breeder adjusting to that is really [0.3] quite difficult [0.5] okay [0.4] so you have to do some crystal ball gazing and it's difficult to get it right [0.2] you have to bear that in mind so of course one of your strategies [0.3] may be [0.2] to put some adaptability into your breeding programme so that you can [0.2] move in different directions not just [0.3] selecting for a single [0.5] er [0.2] particular picture [1.9] another question that you might [0.4] er try and ask as a plant breeder is what is going to be the spectrum of [0.4] pests and diseases [0.4] in seven years' time [0.3] and i can assure you these do [0.2] move and do change [0.5] quite a lot over [0.4] time [0.6] er certainly [0.4] we produced varieties that by the time we got them to the field [0.4] were susceptible to one of the most common diseases around and they were useless [1.5] so [0.6] the reality of predicting is difficult but you do need to do something about it [2.3] okay so they're real problems in terms of [0.2] the breeder defining [0.4] the aims and objectives [0.3] but if we ignore that [0.9] the next question is [0.3] really how do we go about [0.3] trying to achieve these aims and objectives [0.4] and that's really what what the course is about [1.9] now i'm sure [0.2] you've all got some [0.2] reasonable ideas from the lectures you've already [0.4] er [0.4] been to [0.2] about the some of the general features of [0.2] plant breeding [1. 2] but basically one of you can break it down [0.4] into [1.9] really three steps [3.2] and these are common [0.6] to [0.7] every breeding programme [1.1] now the details of them may differ [1.3] but [0.4] nevertheless [0.3] you require all these [1.1] all these three steps to be able to [0.2] to to carry out plant breeding [6.3] the first thing [0.3] you need to do is to either release or produce genetic variation [0.6] if you want to select for something that's different [0.3] you've got to have a difference there to start with [0. 9] if they're all the same then clearly there's no point doing anything [1.2] now to do that as i say you need variation that is genetical [0.2] you need things that are inherited [1.2] the two things may look different [0.3] and by that we say the phenotypes are different in other words their appearance is different [0.9] but they need to be genetically different if it's going to be inherited by their offspring and therefore we make progress with plant breeding [0.8] so we need [0.4] to be able [0.2] to produce and release genetic variation [2.2] and i'm sure you're [0.3] all know [0.8] very well what is the most [0.6] obvious and straightforward [0.3] way to produce [0.2] genetic variation [2.5] and it is [1.2] [laughter] [2.6] no sm0189: [0.2] nm0187: sorry [0.6] sm0189: it's hybrids [0.3] nm0187: yeah [0.4] sexual reproduction [1.2] er i-, [0.2] i-, [0.2] i-, so yeah it is a very obvious answer [0.4] so sexual reproduction [0.3] is the most common [0.4] er [0.4] form of [1.5] releasing variation in plant breeding [0.4] and in [0.7] virtually all plant breeding programmes that is the source [0.8] of genetic variation [1.5] so we take [1.2] two lines [0.3] which have [0.2] between them the characters [0.3] or the expressions of the characters we want [0.3] we cross them [1.1] we produce [0.3] usually the first generation from that [0.5] you then [1.1] have various options but one of the obvious things to do is to sell [0.6] er [0.4] that [0.5] to produce [1.0] segregation [1.8] so we [0.6] get the differences to segregate out and we try then to select the ones which have [0.3] the right combination [0.4] characters all the plus [0.4] points together [0.3] in one [0.3] in one particular genotype [0.9] in other words we look for recombinants [1.2] okay [1.2] er [0. 5] it sounds i know very simplistic but that [0.3] is basically [0.4] in w-, in most plant breeding programmes what you're trying to do [0.8] you're trying to put together the characters from different lines into one line [0.4] you have to identify it [1.0] er [0.3] and therefore do something with it [1.7] okay so that covers the second part in a way [0.3] we need to produce the variation we need to select amongst it [0.8] okay [2.3] when we have selected amongst it we need to stabilize it to do something with it [1.8] we're going back up to here [1.4] sexual reproduction is the commonest [0.4] way of producing the variation [0.3] but it's not the only way [1.2] there are other possibilities [0.6] one is to induce mutations [0.4] and we'll talk a bit about [0.3] that and you can do this [0.3] in various ways mutations are naturally-occurring events [0.3] but you can increase their frequency [0.2] by using a mutagen [0.5] ionizing radiation for example [0.3] here's one [1.0] and this is used in plant breeding in a number of crops quite a few of the clonally-reproduced crops like apples for example [0.6] mutations [0.2] er [0.4] are used and we'll discuss that a bit in the [0.8] in the course [1.3] we can also try [0.3] and although it's in some ways it's sexual reproduction it usually needs some [0.3] help [0.2] we can use [0.3] wild ancestors or related species [0.2] to cross with our [0.3] cultivated [0.8] species to [0.3] increase the amount of variation we have available [0.3] to try and perhaps [0.2] introduce characters where we don't seem to have variation [0.6] in the domesticated forms [1.5] and the other possibility of course now is the newer techniques such as for instance genetic transformation [0.6] genetic manipulation whichever you want to call it [0.3] and of course that provides us another [0.4] possibility [0.4] in this release production of variation [7.5] really whatever [0.4] source of variation [0.3] we use though the im-, or or [0.2] probably the most important [0.4] er criteria is the selection of the parents [2.7] unless you selected what parents to start with [0.3] you've already got [0.3] a major uphill [0.3] battle [12.7] so on here [0.2] i've written it as the critical factor [1.6] okay [0.8] so unless you have [0.4] good starting point you're always going to be [0.3] struggling [0.3] so you need to choose [1.4] the parent [0.3] and that might sound [0.5] relatively easy and it can be [0.4] if for example [0.2] you use a cultivar that's been widely grown so [0.3] things are known about its performance [1.5] because the difficulty and i hope this is not [1.6] a [0.2] completely new concept to you you should have covered it before [0.3] but what we see [0.8] is the phenotype [1.5] okay [0.2] so what i'm [0.2] looking at in terms of seeing you is your phenotypes [1.2] okay [0.5] it's what you see is the phenotype [0.5] the phenotype [0.8] is made up [0.6] of [0.6] the genotype [0.7] what genes you've got [0.3] and how they're being expressed [0.4] in other words what's [0.2] inherited [0.5] from your parents [1.0] is being [0.2] [0.4] but it's also [0.4] a product of your environment [1. 7] okay [0.8] what conditions you grow in what food you've had all these things which determine [0.5] er [1.3] then [0.2] how you appear [1.0] you can easily for instance affect people's height [0.4] by their nutrition or at least relatively easily so you only have to look at their history [0.8] the other complicated factor is that the genotype interacts with the environment [0.6] different people given exactly the same food react differently [0.5] er [0.2] different conditions et cetera [0.6] okay [0.4] so [0.5] when we look at something unfortunately [0.3] we have this complex of underlying factors [1.1] and the thing that's critical to us as a plant breeder [0.2] okay we can exploit the [0.2] interactions [0.3] geno times env [0.2] but really what we're interested in is this part [1.8] okay [0.7] but these other things can be [1.2] major and mask [0.6] which bit is genetic and you don't know [1.1] so [0.3] when we're talking about the choice of parent this is a complicating [0.2] factor and it's a a factor that will come in [0.3] elsewhere as well when we [0.3] are looking at this er [0.7] but this business of wanting the genotype be-, [0.2] but only being able to [0. 3] observe the phenotype is a [0.4] is a major drawback [1.6] okay [2.4] so [2. 2] having done that we've chosen the parents we've [0.2] done something about releasing the variation [0.2] probably sexual reproduction but [0.6] could be other things [0.4] we now need to select amongst the variation [3.0] again [0. 5] on first thought that seems [0.2] kind of easy [0.3] just go and have a look see what's good and take it and all the rest of it [0.7] but the first thing you need to decide is what characters [0.3] are going to be selected [2.2] again this seems fairly straightforward [1.4] but first of all you have to realize that many characters are actually quite difficult to measure [0.8] they either take a lot of time a lot of energy [0.5] expensive equipment [0.5] er [0.8] or really just a very difficult t-, to do the second [0.3] er [1.4] thing [0.4] you need to bear in mind is that you've got a lot of material to look at [0.4] a lot of different ones that you want to assess [1.4] right something we'll come to again [0.3] in the [0.2] course [0.5] and the other problem is that you usually have small quantities of it [1.0] okay [0.2] so for instance i mean taking the very obvious thing [0.7] you might have two plants how are you going to assess yield [1.3] yield of two plants grown by themselves doesn't er [0.8] doesn't tell you anything very much it's nothing about [0.3] er [0.3] what it'll what it'll actually [0.2] er [0.2] grow like [0.7] and also for disease [1.1] for instance [0.5] so quite often er [0.4] for disease assessment they will take [0.2] er [0.4] detached leaves [1.6] because there simply isn't enough material [0.5] you cannot have different densities of sowing you cannot [0.2] er [0.2] plant material [0.2] er [1.1] that er [0.3] can be grown exactly [0.2] as you would like [0.7] in agriculture [0.4] so [0.8] the question is [1.1] how are you going to reproduce the conditions of agriculture [0.3] you can't totally so you have to pick a single condition [0. 5] and work on that basis and hope it's representative [0.5] you cannot [0.2] as i say grow large plots and see how they behave [0.4] you've only got a small amount of material [1.8] a small amount of material also means that you have questions about the relevance [0.7] of what you're measuring [0.3] and the characters you're assessing to agriculture itself [0.9] so you can talk to pathologists and they can tell you how they can take [0.4] single leaves from plants they can expose them to diseases [0.6] and they get a reaction to it [1. 3] but this doesn't always tie up with field performance [0.7] so [0.3] the relevance [0.2] of the characters as well [0.3] as the effectiveness that you can measure them [0.2] is extremely important [4.5] the next problem we have [0. 8] is really the one that we were talking about a few minutes ago and i said it would come back up [0.5] and that is the efficiency with which we can select [2. 1] so back to the phenotype versus the genotype [1.7] [cough] [4.0] as i think is fairly obvious from th-, [0.2] from the equation [0.5] the greater the environmental effects [0.4] the more the phenotype [0.8] will be a poor reflection of the underlying genotype [1.1] okay [0.4] so the more something's affected by the environment [0.2] the less we can do anything about [0.5] er [0.2] picking it because we won't be picking the genotype at all we'll just be picking the effect of the environment all the time [1.8] so this creates [0.4] er [0.2] a real problem particularly as [0.2] many of the characters we're dealing with are not the simple ones that Mendel had you don't get [0.5] tall and short [0.4] pink or white [1.3] these sorts of characters we get [0.3] characters [0.2] like yield [0.3] like number of ears [0.6] so if you think for instance [0.2] the character that's similar in humans is height [0.8] you are all different heights [0.9] but [0.3] the fact there's not one of you here that's a major g-, has a major gene difference for height in other words there's no achondroplasic dwarves [1.0] but [0.2] they do occur [0.3] in the population there are major genes for height [0.4] but most of you differ [0.3] for height [0.6] by a lot of genes [0.7] there's a lot of different genes will affect your height [0.8] and so what we're [0.3] seeing is the effect of all the genes okay plus the environment and all the rest of it [0.5] but [0.6] there's a lot of genes there so it's not easy [0.6] to say what's genotype and what's phenotype [1.1] okay [1.6] [cough] [1.0] so to try and get out of some of those problem we have to find ways of trying to get some sort of assessment [0.3] of what amount of the variation [0.3] might be genetic and what might be [0.4] er environmental [0.2] and we can get a measure of something [0.2] called the heritability again which we'll [0.3] come to later [0.4] in the lecture course [0.3] which effectively just measures the proportion of the variation or tries to [1.0] of the total variation that's genetic [0.7] so it gives us some sort of estimate of how efficient [0.3] or how effective our selection's going to be [2.2] [cough] [4.1] it's something i'll talk about when we talk about heritability [0.3] and you get various discussions of this mostly in [0.3] er [0.4] not [0.2] perhaps quite so much recently but [0.2] er not too long ago about I-Q in humans of what heritability was and [0.3] what this meant and how this affected racism and all the rest of it [0.6] the thing you have to remember is there's no such thing as the heritability [0.4] the heritability is only estimated for a particular population a particular point in time [0.6] er so there's no [0.3] there's no general [0.2] measure for it [1.2] but it gives us some [0.3] some idea about how [0.3] er [1.1] how we might proceed and the reason i'm stressing that it changes is because we can change it as plant breeders [0.8] we change it because we're doing the selection but we can also change it by the way we carry out our trials [0.6] if we replicate things [1.0] okay [0.6] we replicate things we can take the mean [0.3] if we take the mean we know it more precisely [0.4] the more observations that go into the mean the more precisely we know [0. 3] and the more precisely we know it [0.3] the more [0.5] we can increase the heritability [1.0] okay we reduce the environmental effects [1.0] compar-, compared to the genotypic effects [1.3] now you can do this in various ways you can simply replicate the trials so that [0.6] decreases the local environmental variation [0.7] we could replicate it over different trial sites [0.2] so we start to change [0.4] the [0.4] how sure we are about its repeatability [0.3] over different regions we can do it in different countries we can do it in different seasons [1.0] okay [0.5] so we can change [0.8] the heritability [0.4] and we can also change the repeatability of our measurements but [0.9] [cough] [1.1] but [0.2] the trouble is each time we do that we increase the workload [1.3] so [0.2] if we have two replicates we've already doubled [0.2] the amount of material we have to grow [2.1] you then grow it at another site you've doubled it again [1.7] usually there is a very [0.5] real limit on how much you can cope with or how much you can grow or how much seed you've got [0.6] so [0.2] normally what it means is that you [0.9] look at less material [0.4] so if you double the number of replicates you halve the amount of [0.6] lines you look at [0.8] so you gain on one thing and you lose on another [1.1] okay [0.5] so it's again it's something we need to consider as we [0.3] er [0.4] go through the course that this is a [0.3] a problem we can increase precision in one way but if we do that [0.4] we [0.8] then reduce how much material we can look at and we then reduce the [0.3] efficiency of selection [2.3] [cough] [3.7] [hum] [2.7] okay but we carry out our selection in ways that we'll [0.2] er see as we go through the course [0.5] having done that [0.6] you end up [0.7] with an amount of material [0.5] which you need to stabilize you need to be able to repo-, reproduce [0.4] this material if it's going to be exploited in [0.4] er [0.6] any way at all [1.3] and you need to multiply it [0.8] so you'd need to multiply it stably [1.7] okay otherwise [0. 3] i mean what's the point of having one seed which is just what you want what can you do with it [1.1] [cough] [1.0] now this is where the different types [0. 3] of [0.3] crop plants [0.2] er [0.6] tend to be more different [0.4] the main [0.6] er [0.3] criteria that are used for separating plants in terms of plant breeding [0.3] are to do with their breeding system [0.7] and three main [0.4] er [0.5] types [0.4] that are recognized are [0.8] inbreeding [0.2] outbreeding and vegetatively reproduced crops [1.0] and we'll go into these in er [0.2] in in the next lecture [0.4] they [0.2] i think are fairly self-explanatory [0.8] inbred crops [0.3] mean that they can be selfed [0.4] you can [0.2] therefore [0.3] make them true breeding [0.3] therefore of course you can multiply them without too much problem [1.1] vegeti-, [0.2] vegetatively reproduced crops [0. 4] are ones [0.2] which you reproduce asexually [0.3] and therefore you can multiply them up without changing their genotypes at all [0.7] potatoes are a good example they produce tubers you plant a tuber you get more tubers [0.3] they're all the same genotype [0.9] so they're not too much of a problem [0.5] the outbred ones [0.3] [laugh] we need to talk about as we go along [0.3] er more these are really [0.4] because they're all genetically different [0.2] you need to treat them more as a population so you try and keep the population the same [0.3] rather than the individuals [0.8] okay [0.3] so with inbreeders we can [0.5] have [0.2] individuals which are exactly identical [1.0] for the outbreeders it's really the populations of the things that need to be stable [0.3] and you have to have ways of doing that [1.5] [cough] [3.9] okay so that's [0.8] really i think [0.5] as much as i want to say [0.8] on that part i don't know if there are any questions [0.4] so [0.3] as you might [0.2] guess now if you look back [0.3] the next three weeks [0.2] what i'll try and do is cover those three main groups to give you [0.3] the basis [0.2] of the breeding of the different [0.6] groups and then we'll go on to other things [1. 5] okay [0.7] so any [2.1] questions i've [0.5] completely confused you now [0. 5] [laughter] [2.1] no [0.7] sm0188: er you have one question [1.0] er can we just last minute [0.4] here's the wheat [0.2] in Germany [1.3] and [0.2] look like a grass but has roots [0. 9] and from the roots there comes another [0.3] nm0187: yeah [0.4] sm0188: and then it's like a potato common crop right nm0187: yes [0.4] yeah [0.8] yeah i er [0.3] sm0188: i know nm0187: i'm not sure whether it's it's wheat or not but i mean i-, it it can be from the same family but there are [0.3] quite a number of plant species certainly a number of the grasses [0.3] that reproduce by stolons [0.5] er [0. 2] the other way that you can get clonal crops of course is to take cuttings [0. 4] sm0188: just like apples yeah nm0187: apples is a good example [0.4] you take cuttings put it onto a rootstock [0.7] and you can reproduce them [0.4] er [0.2] various ways as is say there are quite a lot of [0.2] er things sugar cane [0.7] you cut sugar cane down chop it into pieces plant the pieces and they all grow [1.3] yeah [0.7] ornamental c-, er plants quite a lot of them are [0.3] vegetatively reproduced [1.5] yeah [3.3] okay well if there are questions i mean c-, ask any time [0.3] about them [0.2] what i'd like to do [0.7] then if we cover that is to tell you a bit more about the posters [0. 4] and this sort of thing [0.3] er [0.9] yeah [0.2] is that okay [1.4] right [1.6] oh no one more thing [0.7] sorry reading [1.1] list [0.2] there's a list of er [1.6] they're really there's just books 'cause these are [0.6] are general [2.2] i don't s-, suggest for one minute you have to go away and read all these books [0.5] or you necessarily have to read [0.3] one of them all the way through [0.3] but if you're looking [0.3] er [0.9] to cover the more general things then [0.7] books are about the best way of er [0.3] of doing it [0.3] er it's up to you [0.2] sh-, [0.2] i put all of these [0.9] i think all apart from the first one on seven day [0.6] loan that's not to [0.3] restrict you and i hope it doesn't cause you too much problems but it seemed the fairest way of making sure [0.3] people could all access them otherwise the first person there takes the books out and [0.3] that's it for the term [0.4] okay [0.3] er [0.3] and there are a number of copies of er a number of them [0.7] it's it's really up to you which ones er you want if you want to talk about them at all i i can do but i i just tried to give you some of the general [0.4] er [1.0] books the book in fact by [0.6] Bosemark is probably the more specific or the more er [0. 6] restricted [0.2] of the other ones the other ones are more general than that [0.7] and that's why it was written [0.8] to be more restrictive [0.2] yeah [0. 9] okay [2.3] good right [3.8] more paper [0.5] hopefully [0.4] most weeks there won't be so much paper but [5.6] [hum] [5.0] okay so the first thing [0. 8] that of course you need to do [1.8] is to pick [0.6] an area [0.5] or a topic [0.4] on which [0.4] you're going to [0.4] do the necessary reading find out about things and [0.6] then [0.9] produce [0.2] your poster and essay [2.4] okay [0.2] now these are just ideas they're not [0.4] they're not the only things [0.3] you can do but i thought rather than [0.4] present you with a [0. 2] you know it's like at school they tell you can write an essay on anything and that's the worst possible [0.2] situation [0.4] so these are ideas clearly it's got to be a restricted area i mean you know you can't talk about er [0.3] you know [0.3] plant breeding in general or er [0.5] micropropagating plants in a you need to to talk about say micropropagation of strawberries or [0.4] you know [0.5] breeding [0.5] sugar cane for [0.3] drought tolerance [0.3] y-, y-, you need a fairly restricted area [0.3] and that's why i want to agree with you what the topic is so that A it's not impossible for you to do [0.3] for being too [0.3] wide [0.3] but on the other hand [0.3] there's enough chance of you finding some literature [0.3] and something to say about it a [0.4] kind of [0. 3] blank at the end is not going to be very useful [0.6] okay [0.5] so [0.5] have a think about it i mean clearly to to a certain extent it depends on what your interests are [0.4] er [0.5] and what have you [0.2] and hopefully we can come to [0.2] an even better topic which you can find enough about [0.3] you can present it and i say what [0.4] er we want you to do is to produce the poster and the essay which okay [0.3] is part of the assessment [0.3] but also [0.3] other people can look at you can answer [0.3] questions about it and [0.4] all learn from each other [0.9] okay [1.5] so to do that [2.3] just so that i can th-, [0.4] also remember what the [0.4] there was happening [0.3] there's er [1. 1] you need to [1.0] fill in a form one part [3.2] i'll keep and one part you'll keep [0.2] so we need to do that probably [0.2] er [1.2] whenever you wish but by [0.6] er [0.3] certainly by the end of next week [0.7] need to not because i want to press you about it but clearly [0.3] if i leave it long making the decision then you're not going to have much time to actually do the [0.5] to do the er [0.2] research and produce the material [0.4] okay [0.8] so we need to decide fairly [0.6] fairly quickly if you want to come and see me in the week or anything that's fine [0.5] er [0.4] but have a s-, about it [0.3] have quick [0.3] er [0.5] chat and if it's okay then you can go ahead on it [0. 8] all right [0.2] sm0189: so any of these er [0.3] categories here you can just see just select this the number of these [0.2] categories [0.5] nm0187: yeah you cou-, y-, yes sm0189: nm0187: i mean it's just to give you a yes yeah yeah so i mean you can just pick on to know for instance [0.2] transformation for drought resistance or er sm0189: yeah so you wouldn't use a number you'd just use one of them or nm0187: you'd use one of them or part of them i mean s-, s-, silly i mean [0.2] you know a [0.2] a a topic called storage sm0189: right okay nm0187: is much too wide you know storage of apples or stora-, sm0189: yeah nm0187: i [0.5] i just put down ideas to give something to to to start with sm0189: [0.3] nm0187: yeah [1.0] i mean do think about the reality of how much [0.3] you're going to read about this and how much you're going to get written down sm0189: yeah nm0187: to make it sensible [0.2] yeah [1.0] okay [0.6] now to go a bit further 'cause in fairness to you [1.0] you need to know [0.6] what's [0.3] important in [0.7] producing your [0.3] poster [0.7] and i'd rather tell you about it [0.5] now [0.3] so that you know when you're doing it [1.7] rather than spe-, [1.3] spring it on you at the end [1.3] okay [0.2] so [0.5] as i say you'll present your [0.2] poster [0.2] to all the other people i want each of you [0.2] then to mark [0.8] or give assessments of everybody else's things [0.3] now this is not for you to be nasty to each other [laughter] and it's no good then saying everybody's i-, is an A [0.4] er [0.2] i want you to be realistic obviously [0.2] and [0.7] i've suggested here the criteria that we'll use for this [1.2] there are th-, beally three [0.2] parts to [0.4] the poster [0.5] first of all is the general appearance and presentation of it [0.5] er [0.5] if you ever go to or you probably will start going to scientific meetings [0.3] the [0.2] the first thing is there's you know there may be a hundred of them there [0.5] you're not going to look at all of them so the first thing you do is just got to see which ones look nice or pretty or [0.2] something you know anything which will [0.2] which will attract you [0.2] and so you go to it [0.2] okay so [0.4] i-, you know they don't have to be pretty pretty or they don't have to be wild or anything but they must be [0.3] visually [0.4] reasonable and p-, have some reason for you would want to read it [0.2] you know there's lots of tiny little writing and nothing much there you you actually don't spend much time with them [0.7] okay [0.4] then [0.2] when you get that far the next important thing is the content and the information that it [0.8] gives to you [0.6] okay [0.5] now you should remember with a poster [0.3] that [0.3] and this is the difference between your poster and your essay [0.5] the poster should be enough to interest people [0. 2] and to make them want to ask you some more questions and be intelligible [0. 3] they don't necessarily cover every last bit of the subject [0.9] okay [0.4] because there really is a limit to how many words and things you should put on the poster [0.4] it really should be [0.2] something that visually interests people and [0.4] gets them interested [0.4] rather than answer every question [0.6] okay so don't be tempted to try and [0.6] cover every possible thing you found out about the subject cram it on to your poster [1.1] okay [1.2] yeah [2.1] then [0.3] the next part or the last part is is background knowledge and this is where [0.4] er [0.4] really [0.2] i say [0.2] i want [0.5] the rest of you to ask questions er but not t-, to try and catch them out [0.3] but just to make sure you've understood what they've said [0.3] perhaps because [0.3] what they said is quite interesting and all the rest of it and so [0.4] er [0.2] that's what that part is so that's the three [0.4] er [0.3] major [0.3] er [0.9] parts that will be [0.8] assessed and i tried to [0.3] er [0.6] on the back of the sheet [0.4] take out the assessment criteria so that [0.3] er [0.4] you go from A to E all the time so we keep it fairly consistent and give some ideas [1.1] so for appearance and presentation A represents an excellent well presented poster wherea-, E one is untidy poorly conceived with little appeal [0.6] okay [0.3] i mean it's perf-, [0.3] i mean they're not [laugh] these are not strictly defined criteria but to give you a kind of idea so the same for [0.2] content and information [0.3] A is an informative poster which you gained an interest with a satisfactory amount and level of information [0.6] whereas E is a poster from which you gained little [0.2] if any useful information and not really any interest [1.0] yeah [1.2] and for the background knowledge [0.3] represents a well informed presenter whereas the presenter that has very little background knowledge [0.6] so okay they [0.4] put the things down on the poster but [0.2] that's about [laugh] all they can manage [0.7] yeah [0.6] is that [0.3] okay i know it sounds a bit crude but i-, it's it's a [0.4] if you s-, bear that in mind as a sort of criteria i think it'll become fairly obvious and [0.5] it'll be fairly [0.6] easy to do it yeah [1.3] sm0188: going to be a question about the poster [0.5] nm0187: about the what [0.3] sm0188: about the poster nm0187: yeah [0.2] sm0188: er [2.8] could be er [0.3] like a and [0.2] er [3.1] but it's not er [0. 4] the [0.2] for example there is [0.9] there's a breeding sheep on it and [0. 2] afterwards you tell people how it's going or [1.5] nm0187: well that's part of how [0.2] and what i want you to think about about designing the the poster [0.9] if you just put on something with no explanation [1.4] people are not going to get anything from it [0.2] i mean you've got okay you're going to be there to answer questions but the poster has got to be free-standing [0.9] it's going to be there and people can look at it and say oh yes you know that's u- huh [0.3] i see that's interesting i wonder what [1.5] et cetera [0.7] okay [0. 2] so [0.2] it's getting a balance between having it [0.3] attractive enough to look at and enough information [0.6] to convey to people the basics of the subject area what it's about [0.3] what happens what's achieved [1.5] yeah [0. 4] but not necessarily covering [0.7] all the things it doesn't matter that you [0.5] need to produce twenty lines to do this the fact that you've used some lines is okay rather than [0.4] specifically twenty [1.8] i-, [0.2] it's something i think we'll have to see a little bit as it goes along you've got a few weeks to [0.3] to do it till you get some help but it [0.2] it is that balance is the difficult thing between [0.2] just providing loads and loads of information which i-, is very relevant but is [0.3] usually boring and not very er [0.8] easy to take in [0.5] and providing something which is just one colourful picture and actually [0.4] you've really got no idea till somebody tells you what it's about and what's achieved and how it gets done [0. 5] sm0188: is it a little bit like er produce [0.2] this [0.3] for example with Powerpoint [0.2] nm0187: yeah [0.2] you are going to use Powerpoint [laugh] t-, to produce it so [laugh] er but it'll be a single [0.3] it'll be a single page with images on it [0.2] if you imagine [0.4] at least a me-, we will actually or i'll t-, [0.2] try [0.5] er [0.2] to get them printed [0.5] er for you so you've got a printed poster at the end [0.3] and if you think of them [0.3] basically well we'll tell you in the next few weeks but basically like that one board on the wall [0. 9] you have to produce something which has the images and writing on it that's that size [0.6] including the title your name and [0.5] you know these sorts of things [1.1] yeah [0.4] so what you're aiming to do is produce something physically [0.3] like that [0.2] okay quite a lot of the time you'll do it electronically but [0.2] you've got to see it [0.6] as that [0.8] and what we'll basically do is produce that er [0.2] and also probably what we'll do is put it onto an acetate sheet so you'll [0.3] blow it up like this actually to to talk to [0.2] because it's a bit easier for me to handle it this way sm0189: have you got an old one of these posters just so we can have a look at it and so nm0187: yeah sm0189: is that all right nm0187: yeah sm0189: and we did say we'd give you numbers nm0187: yeah sm0189: and then nm0187: yeah [0.9] yeah [0.3] er what i've [0.2] [laugh] [0.4] what i've do-, i'll let me hand out this [0.2] it'll be easier if i t-, [0.3] talk about what i'm [0.8] really thinking about [0.2] doing [0.7] this is the timetable [0.3] er so f-, forget the other one this is what i'm [1.1] we shall be working to [2. 3] so this week i've [0.3] done a bit of handing out the [0.3] the lists and the forms and you've got them and you need to start thinking [0.5] er [1.5] next week [0.3] if you've made the choice [0.2] then you can start doing [0.3] some background [0.5] reading and thinking about the subject area [0.8] the week after that [1.0] er [0.4] then in lab A in P-S-L [1.3] yeah [0.7] where the computers are [0.2] sm0189: yeah [0.5] nm0187: then [0.3] i've got two people namex [0.2] namex and er [1.1] namex [0. 7] who [0.4] will help with the [0.4] technical parts of the computer side of things and answer questions and they've also got a sheet about producing the [0. 2] posters and a few tips and these sorts of things [0.5] er [0.4] and that's where you'll start actually [0.6] producing and that's where also [0.3] we'll try and get [0.3] er and er i will remember [0.2] to bring down copies of previous ones so that you can [0.5] sm0189: nm0187: have a look and [0.7] see [0.4] yeah in fact er i only if i remember i'll bring them with me next week 'cause i've got some [0.2] on acetate sm0189: yeah nm0187: so we can just [0.4] quickly look at them here sm0189: yeah [0.2] nm0187: er [0.2] sm0188: nm0187: yeah [0.9] but if you go to the [0.2] P-S-L if you walk around the corridors [0.2] sm0189: yeah nm0187: the things that are on the walls [0.4] are what i'm [0.3] talking about you producing [1.0] okay [0.6] so that probably would be the best thing if you had a look at that and i'll try and remember to bring some but if you look at those [0.4] posters they're exactly the sort of thing [0.7] you know i don't say all of them are good [0.6] so don't take it from me [laughter] that i'm saying they're the models for it but then [0.2] they're the sorts of things that you're aiming for the sort of thing that we expect er [0.6] people to do [0.2] yeah [1.1] er [1.3] so [0.5] after week three [0.2] and from week three onward there is [0.3] there are computers booked [0.3] in lab A so that you get priority on them [0.7] and the software's there [0.2] what we'll do is er [1.0] give you each a zip well [0.2] you'll have to give us a deposit but we'll give you a zip disk each so you've got something to work on [0.5] the software's there [1.2] okay [0.4] and then [0.4] you start to go from there [0. 2] and we'll give you what help and advice we [0.6] we can on you know presentation and things like that [0.5] come to week eight [0.4] well week seven you need to hand it in week eight [0.4] we'll then start [0.3] presenting it [0.3] it depends how many there ends up on the [0.4] course last year i only had [0.3] about this number when i started by the next week i had nineteen so [0.4] [laugh] we'll see how [0.3] how many we've got we might [0.3] just do it in one week or two [0.5] okay [0.3] so as i say please don't feel [0.2] nervous about presenting it y-, you know it [0.2] it [0.3] just [0.3] the poster up there [0.2] tell people a little bit about it and they'll ask you questions it's the same for everybody and it's really not a [0.8] a big problem okay [1. 2] er [0.4] and then [0.2] i say we'll make the assessment of it and that's [0. 6] that's it [0.3] and this your essay will be on the same [0.5] subject area so you don't have to go [0.4] revise the whole or you could look up [0.2] information on anything else [1.0] yeah [1.4] is that okay anybody got any [1. 3] sm0190: when does the essay have to be in [0.6] nm0187: er in week eight [5.7] i'm doing that not [0.2] to make you have a short time doing it but [0.4] as you know most assessments [0.9] start piling up at the end of term [0.4] so [0.5] A because we need the posters in then [0. 4] i was [0.5] trying to make it so that the essay [0.3] in at the same time [0. 8] yeah [5.1] all right [2.5] okay [1.7] excellent well if there are any questions [0.5] well most of you do you know where i am in [0.3] P-S-L [0.6] on the [0.2] first floor anyway you'll find me if you ask okay if you want to come and see me [0.7] okay